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KMID : 0387719930040010067
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Korean Journal of Blood Transfusion
1993 Volume.4 No. 1 p.67 ~ p.74
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Optimal Dose of Gamma Irradiation for the Prevention of Transfusion-Associated Graft-versus-host Disease-Effect of irradiation on lymphocytes, platelets and red blood cells
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김대원
강은숙/지현숙/민원기/서철원
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Abstract
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Transfusion-associated graft-versus-ost disease (TA-GVHD) may occur, whenever immunologically competent allogeneic T lymphocytes are transfused to an immunocompromised patient, and in an immunocompetent patient who receive HLA-homozygous blood
for
the
recipient's haplotypes. Irradiation of blood components can effectively prevent the TAGVHD.
To determine the optimal dose of gamma irradiation, functional properties of lymphocytes, platelets and red blood cells were studied following irradiation dose of 500 to 5000 rads. Lymphocyte function was assessed by mitogen (Con-A, PWM and LPS)
stimulated culture with lymphocytes of 5 healthy donor's blood, and platelet function was assessed by platelet aggregation test with agonists (ADP epinephrine, collagen and ristocetin) against 3 donor plateletapheresis components and 4 units of
platelet
concentrates. Effect of irradiation on red blood cell function was assessed with 3 units of whole bloods and 1 unit of packed red blood cells by measuring Hb-ATP, 2,3-DPG, potassium, LD, supernatant Hb, Hb, Hct, MCV, MCH and MCHC.
Irradiated lymphocytes demonstrated significant inhibition of lymphocyte proliferative responses to Con-A from 500 rads (% of inhibition is 68.3%) to 5000 rads (% of inhibition is 98.4%), as compared to nonirradiated lymphocytes.
Clinically acceptable irradiation doses (1.5-15% inhibition to thymidine uptake of nonirradiated lymphocytes lay between 1500 and 5000 rads. ADP-, epinephrine-, and ristocetin-induced platelet aggregation were not affected by exposure to 5000
rads,
but
an impaired response to collagen was noted from 1000 rads in this study.
Gamma irradiation with various dose ranging from 500 to 5000 rads, but an impaired response to collagen was noted from 1000 rads in this study.
Gamma irradiation with various dose ranging from 500 to 5000 rads did not show significant in vitro functional damages to red blood cells (P>0.05).
It can be concluded that a nominal dose of 3000 rads is the optimal amount of gamma irradiation for the prevention of TA-GVHD
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KEYWORD
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